Hello FOURoNers. It's tml! So, I'm doing this as part of my revision haha.
Nutrition/Nutrition in Humans
Biuret Test - Equal Amount of sample and sodium hydroxide. Add 1% Copper (II) Sulphate solution in to the solution, drop by drop. Either a blue or purple colouration. Blue means no protein present while purple means protein present.
Sources of error: Colouration can be subjective to each person.
Iodine test - Just add the iodine to the sample luh. Blue black colouration a indicator of the presence of starch.
Soures of error: Colouration can be subjective to each person.
Benedict's Test: Make a water bath. Put in equal amount of Benedict's solution into each sample before placing into the water bath. Observe the rainbow! Benedict's test is to detect reducing sugars, like maltose and glucose! Sucrose is NOT a reducing sugar
Sources of error: I dunno.
Bile Salts ---> Tested by sinking of sulfur.
If you look at the biology practical skils checklist...
Able to set up apparatus from a diagram or written instructions <---Simple. Able to follow instructions to perform an experiment <--- QED Able to use appropriate equipment to measure:
Volume <---Marks on measuring cylinder. 3 cm^3 is about the length of the first digit of your finger. Temperature <--- use a damned thermometer or datalogger. Take note of the divisions Length <--- ruler. Time <--- Stopwatch
Able to perform experiments using the following techniques
Hydrogen carbonate indicator (what's this?) Iodine test Benedict's test. Carry out a Biuret test Carry out a ethanol emulsion test (Ethanol into test substance. Shake well. Add water. Emulsification means fats present.) Use a microscope to view prepared slides. Prepare slide of temporary mounts. Prepare a specimen for drawing by cutting longitudinal and transverse or staining. Prepare a leaf to test for the presence of starch. Use instruments. blah blah blah.
Basically you must know how to conduct food tests and draw graphs and present your data nicely.
How to draw tables..
Solution Biuret test Ben's test Iodine test Observations and conclusions S1 S2 S3 S4
Something like that.
Since Mr Goh Say bio Spa test skill 1 skill2 is wayang wayang only, so I'd suppose instructions on how to conduct the entire experiment is given. So all we have to worry about is drawing the graphs. Remembering to place units at the headings of our tables. Ensure all lines drawn in pencil and words written in pen. Axes of graphs labelled. Graphs named.
Key sources of error. Target the usual ones. - Colourations can be subjective. Try not to use location of table in lab. Ms Foo say that one not 明显 enough. - Temperature not taking at exact times, as time is needed to read temperature of each sample. Can affect readings of t. Dataloggers can be used instead. I guess that's about all. I can't remember all the other stuff already. :( Wish everyone will achieve full marks at the SPA test on Monday :) It's another step to 10/9A1s.
yongliang
Please be terribly careful and scrutinise the labsheet...
EDIT: Ok since the bio spa is ony chapter 4 and 6...
Remember the food tests. They probably might call you to identify where the substance is. Or they will tell you where the substance is. you conduct the experiment and deduce what the substance is...
so here goes. Mouth: Saliva. Enzymes have proteins. Infer. Stomach: acidic. Enzymes. Protein. digested here. Pancreas, small intestine and gall bladder(I somehow feel that thi part is ging to be the part that will be tested.) : Alkaline. Got pancreatic juices. Alkaline. Digests starch and Fat and protein (everything happens here) Fats emulsified here also.
I guess that's about all. I feeling nervous.
posted by yongliang @ 10:56 am
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